General description hiload 26600 superdex 200 prep grade are prepacked xk columns designed for highresolution preparative gel filtration chromatography. Hiload superdex 75 prep grade data file hiload superdex. Ge healthcare lifescience superdex 200 increase 10300 gl prepacked tricorn columns are designed for small scale preparative purification. Superdex 30, 75, and 200 prep grade media are available prepacked in hiload 1660 and hiload 2660 columns. How to prevent columns from drying out using a syringe duration. Hiload superdex 200 prep grade hiload superdex 200 prep grade has separationrange 0600 000 highestselectivity between 30 000 250000. Purified effectors and cpds in storage buffer 20 mm trishcl, ph 7.
The height of the packed bed affects both resolution and the time taken for elution. Igg pdf structural characterization of vapb46 antitoxin from. Amersham pharmacia ge healthcare hiload and superdex datasheet pdf link to ge healthcare amersham pharmacia website. Superdex 200 increase 5150 gl and superdex 200 increase 10300 gl are prepacked tricorn glass columns. Hiload 16 60 or 26 60 superdeprep grade hiload 16 60 or 26 60 superdex 75 prep grade hiload 16 60 or 26 60 superdex 200 prep grade dismantling tool, support screens, net rings. On hiload2660 column wnt3a will elute at 180210 ml following a 10ml. Collect 5ml fractions for 1660 and 10ml fractions for 2660. Hiload columns for ion exchange and hic are described in a. Superdex 75 5150 gl, superdex 200 increase 5150 gl and. Hiload 16600 or 26600 superdex 30 pg hiload 16600 or 26600 superdex 75 pg hiload 16600 or 26600 superdex 200 pg kav 0. Can anyone help with a selection of gelfiltration column. Hiload 16600 and 26600 superdex 200 prep grade introduction hiload 16600 and 26600 superdex 30 prep grade, hiload 16 600 and.
Hiload superdex 200 pg preparative sec columns cytiva. See also procedure 29140760 for maintenance and cleaning of sec columns. Figure 10 shows mousemonoclonal igg 2b directly from cell supernatant. The ge hiload 1660 superdex 200 prep grade is a prepacked xk column for preparative gel filtration chromatography separations. Artisan technology group is your source for quality. Use for separating mabs from dimers and contaminants. Data file 18116379 ad gel filtration superdex high. Hiload 16600 superdex 75 pg ge healthcare, 28989333. Can anybody help me that which column i should use in place of.
Hiload superdex 30, 75, and 200 prep grade columns bring convenience and high resolution to gel filtration. Superdex 75 prep grade and superdex 200 prep grade are ideal for all stages of an industrial scale. The columns are prepacked glass columns for high performance gel filtration of proteins, peptides, dna fragments which is the best cleaning solution. Hiload superdex 200 preparation grade resin is well suited for rapid, preparative protein purification and scaleup. Size exclusion chromatography the wolfson centre for applied. The molecular mass of the subunit was determined by sdspage. For olecules with molecular weights between 0 and 600000 da. Hiload superdex columns, 200 pg, ge healthcare vwr. A superdex 200 would give you better resolution than a sephacryl.
The eluate was collected in 2ml portions and monitored by the absorbance at 280nm thick line and 540nm thin line. The flow restrictor is not normally used with lowpressure columns such as prepacked hiload, hitrap, and hiprep columns or c and xk columns packed with sepharose based media or other soft media such as sephadex and sephacryl. The column is an excellent choice for separating mabs from dimers and contaminants. Improved purification of histidinetagged proteins with a new imac medium 1847.
Establish a linear flow rate of 60 cmhour, see appendix a through the. Instruction 29027271 ah size exclusion chromatography. Pdf legumain expression, activity and secretion are. Each is available in two column sizes, hiload 16 600 and hiload 26600.
Its selectivity curve gives a broad fractionation range from m r 10 000 to 600 000, with excellent resolving power for antibody monomers, dimers and contaminants of lower molecular weight, such as albumin and transferrin. Superdex gel filtration media are listed in appendix c, table 1. Superdex 75 and 200 5150 gl, with bed heights of 150 mm, a natural column. On hiload1660 column wnt3a will elute at 6580 ml following a 5ml injection. Packing equipment packing reservoir xk1670 column, xk2670. Protein purification chromatography calibration of. Hiload 16600 superdex 200 prep grade are prepacked xk columns designed for highresolution preparative gel filtration chromatography. This amersham ge healthcare superdex 75 prep column is new from surplus stock. Each is available in two column sizes, hiload 16600 and hiload 26600. Crystal structure of cas9 in complex with guide rna and. Calibration of hiload 2660 superdex 200 prep grade. Size exclusion chromatography columns and media selection guide. Ge healthcare hiload 16600 superdex 200 pg hiload 1660.
The column is an excellent choice highly suitable for separating mabs from dimers and contaminants. Hiload 16 600 superdex 200 pg ge healthcare, 28989335, column l. Superdex prep grade pg is produced by covalent bonding of dextran to highly crosslinked agarose. Ge healthcare amersham pharmacia superdex 75 prep grade column. Hiload 1660 or 2660 superdex 30 prep grade transport syringe hiload 1660 or 2660 superdex 75 prep grade hiload 1660 or 2660 superdex 200 prepgrade equilibration before a new run. Something went wrong we were not able to load your agreed msa pricing. Hiload superdex 75 prep grade hiload superdex 200 prep grade fig. Hiload superdex 30, 75 and 200 prep grade columns bring convenience and high resolution to preparative gel. For size exclusion chromatography and analysis of proteins with molecular weights between 10,000 and 600,000.
Glyceraldehyde dehydrogenases from the thermoacidophilic. Hiload 1660 or 2660 superdeprep grade hiload 1660 or 2660 superdex 75 prep grade hiload 1660 or 2660 superdex 200 prep grade dismantling tool, support screens, net rings. Regenerate the column after each run with one column volume of start buffer at. Spare parts hiload 16 600 and hiload 26600 columns. Hiload 16600 superdex 200 pg ge healthcare, 28989335. Instructions 56119099 ae gel filtration media superdex. Ge healthcare hiload 16 600 superdex 200 pg is ideal for highresolution preparative gel filtration chromatography. Influence of sample volume on the resolution of transferrin and igg on prepacked hiload 1660 superdex 200 prep grade. Adjust the adaptor to the medium surface according to instructions for the specific column. Hiload 1660 superdex 200 pg 1 17106901 hiload 1660 superdex 75 pg 1 17106801 hiload 1660 superdex 30 pg 1 171901 related literature poster code no. Ge healthcare hiload 16 600 superdex 200 pg use for separating mabs from dimers and contaminants. Superdex prep grade is a composite matrix of dextran and crosslinked agarose.
Hiload 1660 superdex 200 pg in the second purification step allows for a fast and generic purification protocol that prelimdoes not need optimization. The separation properties of these media are determined by the dextran component high resolution with short run times and good recovery high capacity at high flow rates separations can be scaled up to production levels prepacked for convenience and reproducibility 116 fittings for. Ge healthcare hiload 16600 superdex 200 pg is ideal for highresolution preparative gel filtration chromatography. Fig 4a to hiload 1660 superdex 200 prep grade fig 4b. Introduction hiprep 1660 and 2660 sephacryl s100 high resolution hr, sephacryl s200 hr, sephacryl s300 hr, sephacryl s400 hr and sephacryl s500 hr are prepacked gel filtration columns designed for preparative purification of peptides, proteins and larger molecules. Each is available in two column sizes, hiload 1660 and hiload 2660. The steep selectivity of the dextran component 2 nonspecific interaction 3 column characteristics 4 operation 4 optimization 4 applications 5 hiload superdex 30 pg 5 hiload superdex 75 pg 6 hiload superdex 200 pg 6. Hiload superdex 200 preparation grade resin is well suited for rapid. Size exclusion chromatography ge healthcare life sciences. Hiload 26600 superdex 200 pg ge healthcare, 28989336. Hiload 16600 and 26600 superdex 30 prep grade hiload. With their high physical and chemical stability, very high batchtobatch reproducibility, and regulatory support file backup, superdex 30 prep grade, superdex 75 prep grade and superdex 200 prep grade are ideal for all stages of an industrial scale operation from research and. Hiload 1660 superdex 200 pg removal of dimers andaggregates bufferexchange hchain lchain 3.
Hiload 16600 and 26600 superdex 200 prep grade introduction hiload 16600 and 26600 superdex 30 prep grade, hiload 16600 and. Superdex 200 superdex 75 superdex 200 prep grade superdex 75 prep grade mr logarithmic scale. Close the outlet tubing with the domed nut and then disconnect the inlet tubing. Ge healthcare superdex 200 increase 10300 gl prepacked. Ge healthcare hiload 16600 superdex 200 pg use for separating mabs from dimers and contaminants. The steep selectivity of dextran and the high chemical and physical. Superdex 75 10300 gl and superdex 200 10300 gl are tricorn high performance columns. Elution pattern of mb from a hiload 1660 superdex 200 gel. Ge healthcare amersham pharmacia superdex 75 column in. Superdex prep grade and prepacked hiload columns 1 chromatography media characteristics 2 chemical stability and dextran. Ebola viral glycoprotein bound to its endosomal receptor. Hi all, we have hiload superdex 200 1660 gel filtration column in our lab, which have some sticky proteins and protein.